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Gibco™ 多能干细胞 (PSC) 基因编辑演示试剂盒 A43203货号：A43203 品牌：Thermo Gibco™ 规格：1 kit 
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商品说明
规格产品类型 Pluripotent Stem Cell (PSC) Gene Editing Demo Kit 细胞类型 干细胞（iPS - 诱导型多能干细胞）、干细胞（胚胎） 内容与储存• StemFlex 培养基 (500 mL)，包括： --StemFlex 基础培养基 (450 mL, 2–8°C) --StemFlex 补充剂（50 mL，-5 至 -30°C） • rhLaminin-521（0.1 mg，-5 至 -30°C） • RevitaCell 补充剂（5 mL，-5 至 -30°C） • TrueCut Cas9 蛋白 v2（10 μg，-5 至 -30°C） • TrueGuide 阳性对照品指导 sgRNA，HPRT1（3 nmol；在室温下储存干燥的 RNA，在 -5 至 -30°C 下储存重悬的 RNA；在无核酸酶水或 TE 中重悬） • 基因组 DNA 切割检测试剂盒（1 个试剂盒，-5 至 -30°C） 商品描述
The Pluripotent Stem Cell (PSC) Gene Editing Demo Kit offers the critical reagents for successfully generating and detecting knock-outs and point mutations in your cell line of interest. • Reduce effort and cycle times • Generate pools with high editing efficiency (up to 80% KO and 40% HDR) • Quantitate editing efficiency • Isolate clonal lines easily What you get: • StemFlex Medium (500 mL), comprised of: --StemFlex Basal Medium (450 mL) --StemFlex Supplement (50 mL) • rhLaminin-521 (0.1 mg) • RevitaCell Supplement (5 mL) • TrueCut Cas9 Protein v2 (10 μg) • TrueGuide sgRNA Positive Control, HPRT1 • GeneArt Genomic Cleavage Detection Kit • Manual/protocol (electronic) • Neon demo upon request Efficient genome editing TrueCut Cas9 protein v2 features include: • High DNA cleavage and homology driven repair • Complex with gRNAs into RNPs • Deliver RNPs with Neon system or Lipofectamine Stem reagent Superior single-cell survival The StemFlex/Laminin-521 system with RevitaCell supplement enhances single-cell survival afterelectroporation and single cell sorting. Improved cell survival after electroporation reduces timelines, and the ability to single-cell sort hiPSCs reduces clonal isolation effort and timelines dramatically.
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